Corresponding drugs or vehicles were orally administered once per day for 7 days consecutively.

200 Î¼g/Î¼L LPS was instilled intratracheally in each rat, and then the modeling animals were divided into six groups (n=10, each) randomly: sham group, LPS group, Dexamethasone group, and three different doses (7.5, 15, and 30 mg/kg, i.g.) of total alkaloids-treated groups. Here, we investigated the protective activity of total alkaloids (TA), extracted from the leaves of Alstonia scholaris, against lipopolysaccharide (LPS)-induced airway inflammation (AI) in rats. However, there is no scientific evidence to validate the use of total alkaloids of A. scholaris is used as a traditional herbal medicine for the treatment of respiratory tract inflammation. The formulas including the leaf extract have also been prescribed in hospitals and sold over the retail pharmacies. Zhao, Yun-Li Shang, Jian-Hua Pu, Shi-Biao Wang, Heng-Shan Wang, Bei Liu, Lu Liu, Ya-Ping Shen, Hong-Mei Luo, Xiao-DongĪlstonia scholaris (Apocynaceae) have been traditionally used for treatment of respiratory diseases in 'dai' ethnopharmacy for hundreds years, especially for cough, asthma, phlegm, chronic obstructive pulmonary disease and so on. The kinetics of the antimicrobial activity against PVC biofilm of prepared silver nanoparticles were done using comparative solution suspension time-killing assessments and which are evidenced in Epi-fluorescent microscopic observations.Įffect of total alkaloids from Alstonia scholaris on airway inflammation in rats. As-prepared silver nanoparticles were characterized using the biophysical techniques such as UV-Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, transmission electron microscopy, and dynamic light scattering for the measurement of hydrodynamic diameter and zeta potential. The biosynthesis of silver nanoparticles was done by treating 90 mL of 1 mM AgNO3 aqueous solution with 10 mL of 5 % bark extract. Herein, we used bark extract of Alstonia scholaris one of the most important medicinal plants to synthesize silver nanoparticles (AgNPs) which exhibited excellent antimicrobial properties against biofilm formed in drinking water PVC pipes. Nanobiotechnology is considered as one of the important branches of nanotechnology, and research on synthesis of nanoscale materials, silver in particular, using plant and plant parts has been progressing rapidly. scholaris pentacyclic triterpenoids influence the growth of neighboring weeds by inhibiting seed germination, radicle growth, and functioning of photosystem II.Īntimicrobial kinetics of Alstonia scholaris bark extract-mediated AgNPs scholaris forests, and suppressed weed growth during summer and winter. In the field, ursolic acid accumulated abundantly in the soil in A. Ursolic acid was present in forest soil at a concentration of 3,095Â Î¼g/g, i.e., exceeding the IC50. The triterpenoid concentrations in the leaves, litter, and soil were quantified with liquid chromatography-electrospray ionization/tandem mass spectrometry. pilosa and Lactuca sativa ranged from 78.8Â Î¼M to 735.2Â Î¼M, and ursolic acid inhibited seed germination of B. The half-maximal inhibitory concentration (IC50) for radicle growth of B. The allelochemicals were identified as pentacyclic triterpenoids, including betulinic acid, oleanolic acid, and ursolic acid by using (1)H and (13)C-NMR spectroscopy. scholaris inhibited growth of Bidens pilosa-a weed found growing abundantly near A. We showed that the leaves, litter, and soil from A. scholaris in interactions with neighboring plants.

scholaris, and clarified the role of allelopathic substances from A. In the present study, we identified allelochemicals of A.

However, potential mechanisms-particularly the allelochemicals involved-remain unclear. Alstonia forests frequently lack understory species. Wang, Chao-Min Chen, Hsiao-Ting Li, Tsai-Chi Weng, Jen-Hsien Jhan, Yun-Lian Lin, Shi-Xun Chou, Chang-HungĪlstonia scholaris is a tropical evergreen tree native to South and Southeast Asia. The role of pentacyclic triterpenoids in the allelopathic effects of Alstonia scholaris.

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